Biology

Modern Trends in Molecular Biology

CRISPR-Based Gene Editing
A Solution for Global Food Security

Editors: Shakeel Ahmad, PhD
Asif Ali Khan, PhD
Muhammad Arshad Javed, PhD

CRISPR-Based Gene Editing

In Production
Pub Date: Forthcoming 2025
Hardback Price: $220 US | £170 UK
Hard ISBN: 9781774915981
E-Book ISBN: 9781032712147
Pages: Est. 410pp w/index
Binding Type: Hardback / ebook
Series: Modern Trends in Molecular Biology
Notes: 19 color and 8 b/w illustrations

The discovery of CRISPR has revolutionized crop improvement in agriculture and is emerging as a key factor in addressing global food security challenges. This new volume, CRISPR-Based Gene Editing: A Solution for Global Food Security, highlights the new frontiers and applications of the cutting-edge CRISPR-based gene editing systems in plants and animals for their improvement.

The book begins with an introduction to the CRISPR-based gene editing technique, its discovery, evolution, developments, classification, and diversity. Providing an overview of the new tools in the CRISPR toolbox, the volume reviews the applications of CRISPR-Cas systems in improving plants’ biotic and abiotic stress tolerance, increasing nutritional quality and yield, and more. The book also reviews the application of CRISPR-Cas in the development of desirable livestock and large animals to achieve global food security. In addition, the regulatory aspects and the risks associated with this technology are discussed as well.

With its coverage of research on this state-of-the-art technology, this volume discusses how to harness the power of CRISPR-based gene editing tools for improving food crops and developing climate-smart crops to meet the global food demand in the future. Researchers, faculty, and students in the field of plant biotechnology will find much of value in this new book.

CONTENTS:
Preface

1. 3Ds of Genome Editing Systems: Discovery, Developments, and Diversity
Zulqurnain Khan

2. Application of CRISPR-Cas System in Plant Breeding Technologies
Braulio Edgar Herrera-Cabrera, Víctor Manuel Cetina Alcalá, Luis Germán López-Valdez, Rafael Salgado-Garciglia, Leticia Mónica Sánchez-Herrera, Víctor Manuel Ocaño-Higuera, Jorge Montiel-Montoya, and Hebert Jair Barrales-Cureño

3. Strategies for Delivering CRISPR-Cas Reagents into Plants
Luan Luong Chu and Nguyen Quang Huy

4. Harnessing the Potential of CRISPR Technologies Across the Food Supply Chain
Sameen Shahid, Fidaa Asslam, and Ayesha Imran

5. CRISPR-Based Multiplex Genome Editing in Crop Plants to End Hunger Around the World
Mubeen Fatima, Sidqua Zafar, Kabeer Haneef, Muhammad Ashar Ayub, Syed Muhammad Hassan Askri, Waqar Shafaqat, Rabia Saleem, and Wajid Umar

6. Role of CRISPR-Cas System in the Development of Climate Smart Crops
Zulqurnain Khan, Zulfiqar Ali, and Asif Ali Khan

7. Applications and Explorations of a CRISPR-Based Genome Editing Toolbox in Abiotic Stress Tolerance in Plant
Deepu Pandita

8. CRISPR-Cas Mediated Mutagenesis for Developing Resistance against Plant Pathogens
Khadija Aaliya, Sehrish Iftikhar, Muhamma Naeem Sattar, Farah Saeed, Farah K. Ahmed, Sajid Faiz, Adel A. Rezk, and Kamel A. Abd-Elsalam

9. CRISPR-Cas Mediated Genome Editing in Maize: Present, Past, and Future
Shakra Jamil, Rahil Shahzad, Amina Nisar, Shakeel Ahmad, Muhammad Zaffar Iqbal, Iqrar Ahmad, and Rana Muhammad Atif

10. Harnessing the Potential of Genomic Edition Tools in Soybean Improvement: Progress and Prospects
Muhammad Afzal, Salem Safer Alghamdi, Hussein H. Migdadi, Hira Nawaz, Muhammad Altaf Khan, and Ehab Elharty

11. Use of CRISPR-Cas System for Improving Oilseed Crops: Recent Progress and Future Perspectives
Mei Yan

12. CRISPR-Cas System: A New Toolbox for Developing Transgene-Free Mutants in Plants
Umar Akram, Awais Farooq, Sutar Suhasbharat, Rabia Kalsoom, Furqan Ahmad, Shoaib Ur Rehman, Muhammad Shaban, Waqas Malik, and Zulfiqar Ali

13. Harnessing the Potential of Genome Editing in Large Animals for Food Security: Prospects and Challenges
Ibrahim Bala Salisu, Amina Yaqoob, and Sahar Sadaqat

14. Regulatory Aspects, Safety, and Ethical Issues of Genome Editing in Animals
Muawuz Ijaz, Mubarik Mahmood, Muhammad Kashif Yar, and Iftikhar Hussain Badar

Index


About the Authors / Editors:
Editors: Shakeel Ahmad, PhD
Consultant and Biotechnology Expert, Seed Center and Plant Genetic Resources Bank, Ministry of Environment, Water and Agriculture, Riyadh, Saudi Arabia

Shakeel Ahmad, PhD, is working as Consultant and Biotechnology Expert at the Seed Center and Plant Genetic Resources Bank, Ministry of Environment, Water and Agriculture, Riyadh, Saudi Arabia, where he is also is Principal Investigator of the Laboratory for Plant Genomics and Biotechnology. He and his team are curating the plant gene bank and developing the DNA fingerprints of available plant genetic resources using genomics and molecular tools. His lab is also involved in breeding new, trait-specific, and desirable crop varieties, employing new biotechnological and plant breeding techniques. Dr. Ahmad is also serving as a Focal Person of the Seed Center for international collaborations. He was awarded his PhD degree (Plant Breeding and Genetics/Molecular Biology/Biotechnology) from the Graduate School of Chinese Academy of Agricultural Sciences (GSCAAS), Beijing, China. Then, being one of the pioneer postdoctoral fellows at the Centre for Advanced Studies in Agriculture and Food Security, UAF, Pakistan, he strengthened his expertise in plant genome editing and bioinformatics. He is a recipient of several prestigious scholarships, and in recognition of his contribution to research, the China National Rice Research Institute, GSCAAS, has awarded him the CNRRI Student’s S & T Award 2021. He has published over 20 research articles in international peer-reviewed journals and has also published book chapters. He serves as an editorial board member of the journal Frontiers in Genetics and as reviewer of several high-impact international scientific journals. In addition, Dr. Ahmad is a member and an ambassador of the American Society of Plant Biologists, USA; a member of the Australasian Grain Science Association (AGSA), Australia; The American Phytopathological Society, USA; and a member of Scientific Committee of the Seed Center and Plant Genetic Resources Bank. His research interests include new gene identification, cloning and characterization, gene editing, stress physiology, molecular breeding, and genomics. He is particularly interested in developing climate resilient field crop varieties using next generation sequencing and new molecular plant breeding technologies to achieve zero hunger worldwide.

Asif Ali Khan, PhD
Professor and Vice Chancellor, MNS University of Agriculture, Multan, Pakistan

Asif Ali Khan, PhD, is Professor and Vice Chancellor at the MNS University of Agriculture, Multan, Pakistan. He earned his PhD degree in Breeding and Genomics from the University of Liverpool, UK, and an MBA in Information Management Systems. He also served as a visiting professor at the University of California, Davis, USA. He is an outstanding geneticist and plant breeder and has supervised 17 PhD and over 60 MS students using conventional and molecular plant breeding approaches for crop genetic improvement. He has contributed to commercial and pre-breeding cotton, okra, and spinach. Dr. Khan has also contributed to improving protocol for efficient screening of crop plants for salinity tolerance; protocol for non-radioactive microsatellite enrichment; rapid method of DNA extraction; a low-cost indigenous CEL 1 production protocol; and TILLING platform in cotton. He has authored 13 books, chapters, manuals, and over 150 research articles. He has successfully completed about 20 research, outreach, and capacity building projects with grants from ACIAR, DAAD, USAID, DFID, ICDD, IFPRI, British Council, NSFC, PSF, ALP, HEC, PARB, etc. In addition to developing a new university on a fastest growth model, Dr. Khan has significant accomplishments to his credit for providing services in the agriculture and social sectors development. His accomplishments have been acknowledged by the Higher Education Commission with a Best Teacher Award and by the Government of Pakistan by conferring Civil Award (Tamgha e Imtiaz) in 2020.

Muhammad Arshad Javed, PhD
Professor and Head, Department of Plant Breeding and Genetics, University of the Punjab, Lahore, Pakistan

Muhammad Arshad Javed, PhD, is currently serving as Professor and Head of the Department of Plant Breeding and Genetics at the University of the Punjab, Lahore, Pakistan. He earned his MS and PhD degrees from Kobe University, Japan. He has over 29 years of teaching, research, and development (R&D) experience. His expertise in plant sciences covers molecular genetics, tissue culture, haploid plant breeding, and agronomy. He served Universiti Teknologi Malaysia (UTM), Malaysia (2011–2019). In addition, Prof. Javed has been awarded an excellent performance award (Citra Krishma), including a cash prize, for his excellent performance in teaching, research, and student development. He also received a Konvensyen Felo Award from IPF Malaysia in recognition of his services as a student advisor. He served in the Department of agriculture, Government of the Punjab and developed a fine rice variety “Shaheen Basmati,” which was approved by the Government of Pakistan for general cultivation for normal and saline soils. He has over ten research grants from national and international agencies. He has published over 65 research articles in international peer-reviewed journals and presented over 60 conference papers at different national and international conferences.




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